Journal: EBioMedicine

Article Title: Immune-infiltration based signature as a novel prognostic biomarker in gastrointestinal stromal tumour

doi: 10.1016/j.ebiom.2020.102850

Figure Lengend Snippet: Kaplan-Meier analysis of RFS according to individual TIL populations in the TC. (a) CD3 + TC. (b) CD8 + TC. (c) CD45RO + TC. (d) Foxp3 + TC. (e) CD4 + TC. (f) NKp46 + TC. (g) CD20 + TC.

Article Snippet: Then, sections were incubated with primary antibodies against CD3 (Ventana, 790–4341; ready-to-use), CD8 (Dako, M7103; 1:100 dilution), CD45RO (Origene, TA807197; 1:100 dilution), CD4 (Abcam, ab213215; 1:300 dilution), Foxp3 (Abcam, ab22510; 1:200 dilution), NKp46 (Abcam, ab214468; 1:500 dilution), CD20 (Abcam, ab27093; 1:200 dilution), programmed death-1(PD-1) (Cell signaling Technology, 43,248; 1:50 dilution), and programmed death-ligand 1 (PD-L1) (Dako, M3653; 1:50 dilution) at 4 °C overnight.

Techniques:

Journal: Frontiers in Pharmacology

Article Title: Jianpi Yangzheng Xiaozheng decoction alleviates gastric cancer progression via suppressing exosomal PD-L1

doi: 10.3389/fphar.2023.1159829

Figure Lengend Snippet: JPYZXZ reduced the number of MDSCs in tumor bearing mice. (A,B) Percentage of MDSCs (CD45 + CD11b+Gr1+) in blood determined using flow cytometry. (C,D) Percentage of MDSCs (CD45 + CD11b+Gr1+) in tumors determined using flow cytometry. (E) Immunofluorescence staining for DAPI (nucleus), CD11b, Gr-1, and PD-L1 in tumor tissue sections from the xenograft tumor model. Data are represented as the mean ± SEM, n = 5 mice or n = 3 independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The single-cell suspensions (1 × 10 7 cells/mL) were stained with fluorochrome-coupled antibodies AF488-CD45 (Elabscience, E-AB-F1136L), PE/Dazzle™594-CD11b (BioLegend, 101256), and APC-Gr-1 (BioLegend, 108412) for 30 min at 4°C.

Techniques: Flow Cytometry, Immunofluorescence, Staining

Journal: Cell reports

Article Title: Integration of T helper and BCR signals governs enhanced plasma cell differentiation of memory B cells by regulation of CD45 phosphatase activity

doi: 10.1016/j.celrep.2021.109525

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: APC-Vio770 Recombinant human anti B220(clone REA755) , Miltenyi Biotec , Cat# 130–110-849; RRID:AB_2658286.

Techniques: Negative Control, Recombinant, Purification, Staining, Gene Expression, Lysis, Immunoprecipitation, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Blocking Assay, Cell Isolation, Software, Microscopy

Journal: Frontiers in immunology

Article Title: NLRP3 promotes allergic responses to birch pollen extract in a model of intranasal sensitization.

doi: 10.3389/fimmu.2024.1393819

Figure Lengend Snippet: FIGURE 4 Allergic sensitization to BPE is independent of inflammasome-induced caspase 1 activation. (A) Immunofluorescence staining of lung sections from C57BL/6 and Nlrp3-/- mice (Figure 3) against ASC (red) and CD45 (white). Dapi (blue) was used to stain cell nuclei. Scale bar is 20 µm. The dashed line marks the epithelial cell barrier. b= bronchiole. (B) Quantification of ASC+/CD45+ cells of 3 mice/group, 1 image/mouse as depicted in (A). (C) BPE-specific serum IgE was measured via antigen-induced cross-linking of Fcϵ-receptors on RBL-2H3 cells and subsequent degranulation and release of b-hexosaminidase (b-hex release). (D, E) Cytokines secreted into BALF (D) or by restimulated splenocytes (E) were measured by Multiplex technology. The data are presented as box plots of at least 5 mice per treatment group of 3 individual experiments. Each mouse is depicted as one data point. One-way ANOVA with Tukey’s post hoc test was performed to determine statistical significance. *p<0.05, **p<0.01.

Article Snippet: The following primary antibodies were used: ASC/TMS1 (D2W8U) Rabbit mAb (1:200, RRID: AB_2799736, Cell Signaling Technology), Goat Anti-Mouse CD45 Polyclonal antibody (1:100, RRID: AB_442146, R&D Systems).

Techniques: Activation Assay, Staining, Multiplex Assay

Journal: Cell reports

Article Title: Activation of GPR44 decreases severity of myeloid leukemia via specific targeting of leukemia initiating stem cells

doi: 10.1016/j.celrep.2023.112794

Figure Lengend Snippet:

Article Snippet: Mouse anti-mouse CD45.1 (FITC) , Miltenyi Biotec , Cat# 130–124-211; RRID: AB_2857674.

Techniques: Virus, Recombinant, Enzyme-linked Immunosorbent Assay, Binding Assay, Staining, Modification, Saline, Concentration Assay, Over Expression, Protein Extraction, Membrane, SYBR Green Assay, Bicinchoninic Acid Protein Assay, Protease Inhibitor, CCK-8 Assay, Reverse Transcription, Selection, Plasmid Preparation, Knock-Out, Software, Real-time Polymerase Chain Reaction, Flow Cytometry

Journal: International Journal of Molecular Sciences

Article Title: Cytosine Deaminase-TRAIL Expressing Human Adipose Stem Cells Inhibit Tumor Growth in Castration Resistant Prostate Cancer Bearing Mice with Less Toxicity

doi: 10.3390/ijms27031563

Figure Lengend Snippet: Flow cytometric characterization of parental hTERT-ADSC and ADSC.CD.sTRAIL cells. Representative flow histograms for surface markers. Blue peaks = specific antibody staining; gray peaks = isotype control. Both cell types were strongly positive for MSC markers CD90 and CD105, and negative for hematopoietic markers CD34 and CD45. These results confirm maintenance of the MSC phenotype after immortalization and genetic modification. Abbreviations: ADSC—adipose-derived stem cell.

Article Snippet: Cells were stained with antibodies against canonical MSC surface markers CD29, CD90, and CD105, as well as hematopoietic lineage markers CD34 and CD45 (all antibodies 1:50 dilution; Origene or Invitrogen, Rockville, MD, USA).

Techniques: Staining, Control, Modification, Derivative Assay